The prevalence and distribution of Dirofilaria repens in dogs in the The prevalence and distribution of Dirofilaria repens in dogs in the Mazovian Province of Central-Eastern Poland

Aleksander Wiaczesław Demiaszkiewicz 1, Grażyna Polańczyk 2, Barbara Osińska 3, Anna Maria Pyziel 1, Izabela Kuligowska 1, Jacek Lachowicz 1, Adam Sikorski 4
1 - W. Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland
2 - ‘Megawet’, Veterinary Clinic, Milanówek, Poland
3 - Faculty of Veterinary Medicine, University of Life Sciences, Warsaw, Poland
4 - ‘Prima­Wet’, Veterinary Clinic, Warsaw, Poland
Ann Agric Environ Med
2014; 21 (4):
ICID: 1129918
Article type: Original article
 
 
The aim of this study was to determine the prevalence and distribution of canine dirofilariosis from different districts of the Mazovian Province in central-eastern Poland. 462 dogs aged from 1.5–14 years were examined for dirofilariosis. Blood samples were examined for the presence of microfilariae using the Knott method, as well as the method of Kingston and Morton, after centrifugation in haematocrit microtubes in order to determine the intensity of infection as the number of microfilariae in 60 microliters of blood. The species of microfilariae found were determined after staining on the basis of the morphological characters. Samples were also examined using Canine Heartworm Antigen Test Kit SNAP HTWM (IDEXX, USA) that allows the detection of circulating antigens of females of Dirofilaria immitis. The positive samples were examined using multiplex PCR assay for species confirmation. Microfilariae belonging to the species D. repens were found in the blood samples of dogs originating from the city of Warsaw and from 18 districts of Mazovian Province. The mean prevalence of that species observed in the province was 25.8%. The range of intensity counted with the number of microfilariae found in 60 microliters of blood amounted to between 1 – 150; median intensity 9 microfilariae. The highest prevalence, reaching 52.9% and the highest median intensity of infection reaching 28 microfilariae, was found in Radom district. The lowest prevalence, amounting to 4.2%, was registered in Grójec district; however, the lowest median intensity reaching 6 microfilariae was noted in Żyrardów district. The infected dogs had not been outside of Poland, which means that this is an autochthonous infection. Microfilariae and antigens of D. immitis were not found in any examined blood samples. Results of the presented study show that autochthonous dirofilariosis caused by nematodes D. repens commonly occurs in the area of the Mazovian Province.
DOI: 10.5604/12321966.1129918
PMID 25528905 - click here to show this article in PubMed
  FULL TEXT STATS

Recommend this article to:

Name:
E-mail:
From:
Language:


Related articles in IndexCopernicus™
     Mazovian Province [0 related records]
     autochthoneus infection [0 related records]
     Dogs [2989 related records]
     Dirofilaria repens [8 related records]



 

Related articles

P Umeakuana , B Mohammed ,
Canine Trypanosomosis in the University of Nigeria...
Trypanosomosis is an insect-borne protozoan disease and a serious constraint to livestock production. The incidence of this disease in dogs at the University of Nigeria Veterinary Teaching Hospital (UNNVTH) has not been reported. This study is design...
ABSTRACT
M Felsmann, M Michalski,
Invasive forms of canine endoparasites as a potent...
 Abstract Dogs serve as the vectors of serious zoonotic parasitic diseases. In the month of May 2012 – 2014, 339 dog faeces samples from seven public sites in Chełmno, a town in northern Poland, were collected and examined to determi...
ABSTRACT
J Piekarska, J Bajzert,
Molecular identification of Giardia duodenalis iso...
Introduction. Giardia duodenalis (G. intestinalis) is a common protozoan causing gastrointestinal disorders in many species of mammals. The genus of Giardia has high molecular diversity. Dogs and cats, in addition t...
ABSTRACT